12/28/2023 0 Comments Ceiling vent deflector![]() chinensis ‘Zhaiye Tengben Yuejihua’ × ‘Old Blush’ were used to compare the transcriptome and sRNAome profiles of lateral buds and leaves in adult and juvenile rose seedlings and to detect the molecular mechanism responsible for the extremely short juvenile phase of roses. In this study, 6-month-old F 1 seedlings of Rosa chinensis var. The timing of juvenile-to-adult phase changes are crucial for woody plants, but the molecular mechanisms involved in these changes are still unclear. Structural and mutational analyses have shown that the C-terminal finger and 28 residue carboxyl terminal to the Each GATA factor recognizes a consensus WGATAR motif. A small gene family that encodes GATA factors comprised of two zinc fingers with a structure of Cx 2Cx 17–19Cx 2C has been identified in vertebrates. ![]() GATA factors are transcriptional regulatory proteins that play critical roles in the differentiation of multiple cell types in both vertebrates and invertebrates,. Since GATA motifs are binding sites for a class of C2C2 zinc finger transcription factors in animals and fungi, ,, , we decided to investigate whether an Arabidopsis factor of Discussion In earlier studies, we showed that mutations in the XXIII box, which contains two TATC (reversed GATA) motifs, abolished light induction of GUS reporter in transgenic Arabidopsis plants. The six cis-acting elements that are involved in light regulation of the GAPB gene are illustrated in Fig. A phylogenetic tree based on the amino-acid sequences from the putative DNA binding Results Alignment of the DNA binding domains of these putative GATA factors was performed using the ClustalW program. Twenty-five open reading frames (ORFs) with significant homology were identified. A BLAST search of the Arabidopsis genome sequence was performed using as query the amino-acid sequence of the C2C2 zinc finger of the mouse GATA-1 factor (Accession No. Based on these results, we propose that light controls the expression of GAPA and GAPB genes in part by regulating the binding of the same transcription factor at their GATA motifs.Ĭlassification of Arabidopsis GATA factors. In addition, we showed that GATA-1 could also bind specifically to a cis-acting element in the promoter of the GAPA gene, which is coordinately regulated by light with the GAPB gene. Gel mobility shift assays showed that GATA-1, but not GATA-20, binds specifically to the two GATA motifs within the XXIII fragment. We chose GATA-1 and GATA-20 from Class I and Class II, which include the majority of GATA factors, for overexpression in an Escherichia coli expression system. Based on the degree of the amino-acid sequence identity in the DNA binding domains, we divided the 25 GATA factors encoded in the Arabidopsis genome into three classes. Examination of the sequences indicated that there are two GATA motifs within the XXIII box. We have previously identified a cis-acting element, named the XXIII box, that is essential for light-regulated expression of the nuclear gene GAPB, which encodes the B subunit of chloroplast glyceraldehyde-3-phosphate dehydrogenase from Arabidopsis thaliana.
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